Sellomics | EXPERTS IN TARGET VALIDATION
provitro AG
Provitro’s competence is not limited to the development of protocols for both manual and automated IHC staining but includes, as well, target validation with antibodies on normal and tumour tissue samples which may be provided from our own tissue bank and ISH services.
Sellomics | Your process, your way
Adapt the instrument to the process, not the other way around
Amerging Technologies designs and manufactures in-place Sterilizable Fermenters for various applications:
Our samples:
-> 1.000.000 FFPE -> Ready-to-use TMAs for: i. Normal (off-target) ii. Neoplastic (tumor) iii. Inflammatory + Autoimmune iv. Cardiovascular -> Custom-manufactured TMAs
Our techniques:
-> RNA in situ hybridization -> Immunohistochemistry -> Tissue Microarrays (TMAs) -> Target controls (iConTMA)
Our Services:
-> Paraffin Embedding -> Standard Histological Stainings -> Immunohistochemistry -> Development of new staining protocols -> Digital pathology -> Pathologist evaluation -> Reporting
What are the Advantages of Working with Sellomics?
How we can help you getting your research from good to great
We are a small but experienced team, and take every project individually from the first to the last step
We believe that “possible” is defined by the amount of energy invested. For your priority projects, you need a reliable partner going the extra mile.
Project failure is not an option. If anything happens during the project, we restart at no extra cost and without delay.
Your Partner for Histology and Immunohistochemistry
Histological sections enable precise representation of tissue morphology, with staining outcomes affected by factors like buffer solution pH, staining duration, and fixation techniques.
Immunohistochemical staining, leveraging specific antibodies against tissue antigens, has greatly advanced in precision and breadth. Optimizing factors such as antibody concentration, incubation conditions, and temperature is crucial for high specificity and low background in staining, facilitating accurate tissue analysis.
IHC Services
How does it work?
Provitro’s competence is not limited to the development of protocols for both manual and automated IHC staining but includes, as well, target validation with antibodies on normal and tumour tissue samples which may be provided from our own tissue bank and ISH services.
False-negative results are ruled out by an in-process control system (iConTMA®).
RNA in situ hybridisation
RNAscope® Technology is a novel in situ hybridisation (ISH) assay for detection of target RNA within intact cells. The assay represents a major advance in RNA ISH with its proprietary probe design to amplify target-specific signals but not background noise from non-specific hybridisation. And, the double Z probe design with its relatively short target region allows for successful hybridisation of partially degraded RNA.
Peace of mind in screening and controls
Tissue microarrays are an ideal tool in high-throughput analysis for antigen distribution or validation of newly identified pathogenetic genes.
Tissue microarrays with up to 100 individual tissue samples on a single slide minimise variations in staining intensity, yielding consistent results.
Tissue spots with a diameter of up to 2 mm provide sufficient area for morphological evaluation. Tissue microarrays provide a rapid solution for localisation of DNA or protein molecules in normal and pathological human tissues.
Available TMAs
- Normal TMA (off-target)
- Neoplastic (tumor) TMA
- Inflammatory + autoimmune TMA
- Cardiovascular TMA
- Customised TMA
iConTMA®
iConTMA® are tissue slides for internal control of immunohistochemistry or in situ hybridisation. The slides contain specific positive and negative tissue spots providing enough space to add your tissue under investigation. The specificity of the reaction can be determined and the reactivity can be scored by comparing your sample with the iConTMA® spots.
Process
Manufacturing Process
1st Step
2nd Step
3rd Step
4th Step
Conclusion
For Biology & Pharmaceuticals
Sellomics as a Solution Partner
No drug is born equal, and every project is different. Our team have supported dozens of projects and takes pride in supporting each researcher in their work as they would expect from a colleague.
Step 1
We sign an NDA and make sure we have clarity on goals, budget and timelines. You want to get results soon, we are here to make this happen.
Step 2
With a pilot study you can collect the first results and flexibly evaluate the next steps. Our team will support you in drafting the study and finding the most flexible and cost effective solution.
Step 3
Once the first results are in, you’ll need a reliable partner. Sellomics will help you scale your research and offer a partnership approach to long-term studies that ensures you meet milestones in time and on budget.
